Design and Fabrication of Portable Automatic Tissue Processing Machine
International Journal of Recent Engineering Science (IJRES) | |
|
© 2023 by IJRES Journal | ||
Volume-10 Issue-3 |
||
Year of Publication : 2023 | ||
Authors : Sixtus Amarachukwu Okafor, Innocent Ekuma, Innocent Eze, Uchenna Ezeamaku,Jovita Daniel, Elizabeth Offia-Kalu, Augusta Okafor |
||
DOI : 10.14445/23497157/IJRES-V10I3P113 |
How to Cite?
Sixtus Amarachukwu Okafor, Innocent Ekuma, Innocent Eze, Uchenna Ezeamaku,Jovita Daniel, Elizabeth Offia-Kalu, Augusta Okafor , "Design and Fabrication of Portable Automatic Tissue Processing Machine," International Journal of Recent Engineering Science, vol. 10, no. 3, pp. 84-90, 2023. Crossref, https://doi.org/10.14445/23497157/IJRES-V10I3P113
Abstract
Tissue-sectioning automation can be a resourceful tool in processing anatomical pathology specimens. Microscopic analysis of cells and tissues requires the preparation of very thin, high-quality sections (slices) mounted on glass slides and appropriately stained to demonstrate normal and abnormal structures. In most developing countries, including Nigeria, however, access to this automated equipment is encumbered by a lean healthcare budget. The aim of this study, therefore, is to design and fabricate a portable and cheap automated tissue processing machine with locally sourced materials. The programming sequence used in this study is; Flow chart design., Developing the microcontroller's source code using micro basic language., Debugging the micro basic code, Compiling the source code with a compiler and creating a hex file (i.e. the file extensive is "hex") and Loading the hex file of the programme (Top universal programmer [Top2008]) into the microcontroller's (atmega32) memory. We fabricated an automated tissue processing machine to process tissues between 2-3 mm thick. Comparing slices made from our design and that from a Histokinette ATP300 closure: a conventional tissue processor, we conclude that our design is efficient.
Keywords
Automated, Fabrication, Microcontroller, Processing, Sectioning, Tissue.
Reference
[1] Maristela L Onozato et al., “Evaluation of a Completely Automated Tissue-Sectioning Machine for Paraffin Blocks,” Journal of Clinical Pathology, vol. 66, no. 2, pp. 151-154, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[2] Daniel Jaeger et al., “Label-Free in Vivo Analysis of Intracellular Lipid Droplets in the Oleaginous Microalga Monoraphidium Neglectum by Coherent Raman Scattering Microscopy,” Scientific Reports, vol. 6, 2016.
[CrossRef] [Google Scholar] [Publisher Link]
[3] S. A. Okafor et al., “Miscellany of Hospital Contact Surfaces Microbiome: A Case Study of Selected in Owerri South Eastern Nigeria,” African Journal of Medical Physics, Biomedical Engineering, vol. 8, no. 2, pp. 48-57, 2021.
[Google Scholar] [Publisher Link]
[4] Charles Ferté, Fabrice André, and Jean-Charles Soria, “Molecular Circuits of Solid Tumors: Prognostic and Predictive Tools for Bedside Use,” Nature Reviews Clinical Oncology, vol. 7, pp. 367-380, 2010.
[CrossRef] [Google Scholar] [Publisher Link]
[5] Roberta Galli et al., “Effects of Tissue Fixation on Coherent Anti-Stokes Raman Scattering Images of Brain,” Journal of Biomedical Optics, vol. 19, no. 7, 2013.
[CrossRef] [Google Scholar] [Publisher Link]
[6] Silke Lassmann, and Martin Werner, “Predictive Pathology in Routine Diagnostics of Solid Tumors,” Histology and Histopathology, vol. 27, pp. 289–296, 2012.
[CrossRef] [Publisher Link]
[7] Christian Matthäus et al., “Monitoring Intra-Cellular Lipid Metabolism in Macrophages by Raman- and CARS-Microscopy,” Proceedings of SPIE Photonics Europe, vol. 7715, 2010.
[CrossRef] [Google Scholar] [Publisher Link]
[8] Sixtus A. Okafor et al., “Investigating the Bioburden of “Neglected” Hospital Low Contact Surfaces,” Advances in Microbiology, vol. 12, no. 5, 2022.
[CrossRef] [Google Scholar] [Publisher Link]
[9] Ash A. Alizadeh et al., “Distinct Types of Diffuse Large B-Cell Lymphoma Dentified by Gene Expression Profiling,” Nature, vol. 403, pp. 503–511, 2000.
[CrossRef] [Google Scholar] [Publisher Link]
[10] Yoko Matsuda et al., “Comparison of Fixation Methods for Preservation of Morphology, RNAs, and Proteins from Paraffin-Embedded Human Cancer Cell-Implanted Mouse Models,” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, pp. 68-75, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[11] Sean C. Bendall et al., “Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum,” Science, vol. 332, no. 6030, pp. 687-696, 2011.
[CrossRef] [Google Scholar] [Publisher Link]
[12] William J. Howat, and Beverley A. Wilson, “Tissue Fixation and the Effect of Molecular Fixatives on Downstream Staining Procedures,” Methods, vol. 70, no. 1, pp. 12–19, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[13] Mariana C. Potcoava et al., “Raman and Coherent Anti-Stokes Raman Scattering Microscopy Studies of Changes in Lipid Content and Composition in Hormone-Treated Breast And Prostate Cancer Cells,” Journal of Biomedical Optics, vol. 19, no. 11, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[14] Satoshi Fukumoto, and Toyoshi Fujimoto, “Deformation of Lipid Droplets in Fixed Samples,” Histochemistry and Cell Biology, vol. 118, pp. 423-428, 2002.
[CrossRef] [Google Scholar] [Publisher Link]
[15] Melissa M. Mariani et al., “Impact of Fixation on in Vitro Cell Culture Lines Monitored with Raman Spectroscopy,” Analyst, vol. 134, pp. 1154-1161, 2009.
[CrossRef] [Google Scholar] [Publisher Link]
[16] Michael Titford, “A Short History of Histopathology Technique,” Journal of Histotechnology, vol. 29, no. 2, pp. 99–110, 2006.
[CrossRef] [Google Scholar] [Publisher Link]
[17] Christian Steuwe et al., “CARS based Label-Free Assay for Assessment of Drugs by Monitoring Lipid Droplets in Tumour Cells,” Journal of Biophotonics, vol. 7, no. 11-12, pp. 906-913, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[18] Sixtus Amarachukwu Okafor et al., "Fabrication of a Locally Designed Dual Power Supply Hand – Eye Coordination Tester with a Micro Controller IC Interface," IOSR Journal of Engineering, vol. 12, no. 5, pp. 1-9, 2022.
[Publisher Link]
[19] Sixtus Amarachukwu Okafor et al., “Design and Development of an Adjustable Dynamic Hand Splint,” Engineering and Technology Journal, vol. 7, no. 9, pp. 1449-1458, 2022.
[CrossRef] [Publisher Link]
[20] Kuzmin, A. Pliss, P. N. Prasad, “Changes in Biomolecular Profile in a Single Nucleolus during Cell Fixation,” Analytical Chemistry, vol. 86, no. 21, pp. 10909-10916, 2014.
[CrossRef] [Google Scholar] [Publisher Link]
[21] Geoffrey Rolls, 2019. [Online]. Available: https://www.leicabiosystems.com/pathologyleaders/an-introduction-to-specimen-processing/